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KMID : 0364820080440010043
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Korean Journal of Microbiology
2008 Volume.44 No. 1 p.43 ~ p.48
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Production of Standard Sample for Quality Control of Total Coliform
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Kim Ju-Young
Seo Eun-Yeoung
Kim Mi-Ri
Chung Hyen-Mi
Ahn Tae-Seok
Jeon Nam-Hui
Kim Myeong-Woon
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Abstract
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Standard sample for quality control of total coliform measurement was procured by addition of nalidixic acid and cephalexin as bacteriostatic agents to Escherichia coli cultured broth. After making the standard sample, the number of E. coli was measured by fluorescence microscopic count method and plate count method by 12 hr interval. The numbers of E. coli remained unchanged for at least for 48 hr at room temperature which ranged from 3.5 to 4.2×105 cells/ml and from 1.0 to 1.2×104 CFU/ml by direct fluorescence microscopic count method and plate count method, respectively. This result suggests that microbial standard sample with bacteriostatic agents of nalidixic acid and cephalexin is usable for quantitative quality control.
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KEYWORD
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Escherichia coli, quality control (QC), standard sample, total colifrom
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